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KMID : 0380019940090050457
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 5 p.457 ~ p.461
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Measurement of Cell Concentration of Fungal Broth Using Optical Density
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Abstract
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A new method that considers the pellet sedimentation characteristics for fungal cell concentration measurement was developed using optical density. Appropriate mixing of the pellet suspension almost homogeneously was tried to prevent the sedimentation of the pellet by a small magnetic bar in cuvette, giving a stable optical density. The linear relationship between optical density and the dry cell weight was obtained. Howeve;different curved lines were observed according to the pellet size. Optical density couldn´t be detectable in the size range of 355um above. It was concluded from the result that the use of optical density for measuring cell concentration in fungal broth became possible by considering the pellet characteristics.
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